Wednesday, den 3. February 2010
Bacteria are grown in 15N (heavy) medium and then transferred to 14N (light) medium and allowed to replicate for 2 generations. The DNA is subsequently isolated and centrifuged in a CsCl2 gradient to yield what type of gradient band(s)? a. one heavy band b. one light band c. one half-heavy (intermediate to heavy and light) [...]
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