Laboratory Instruments

Refering to Cheng, H.R., and Jiang, N., (2006) “Extremely rapid extraction of DNA from bacteria and yeast”, Biotechnology Letters, 28: 55-59. I followed most of the protocol, except that I used P:C:I (25:24:1) instead of tris-saturated phenol. 1. 1ml of culture, centrifuged, washed with STE buffer twice. 2. Resuspend cell pellets with TE buffer 3. [...]

Tags: B.subtilis, Can't, Culture, Extract, Just

Refering to Cheng, H.R., and Jiang, N., (2006) “Extremely rapid extraction of DNA from bacteria and yeast”, Biotechnology Letters, 28: 55-59. I followed most of the protocol, except that I used P:C:I (25:24:1) instead of tris-saturated phenol. 1. 1ml of culture, centrifuged, washed with STE buffer twice. 2. Resuspend cell pellets with TE buffer 3. [...]

Tags: B.subtilis, Can't, Culture, Extract, Just

My mom found out today that she has Grade 1 endometrial carcinoma. She is 64 years old. She had a CAT scan three weeks ago, and everything was clear. She had a pap smear 2 weeks ago, and nothing appeared on the cervix. She is going to meet with an oncologist from the Medical College [...]

Tags: Cancer, Diagnosed, Just, Uterine, With

A little about my past. I was on the internet a few month ago and spotted a woman on the “Hot or Not” site, I knew right then that she was a special filly. I finally had several emails with her and we decided that it would be convenient if I moved in the illegal [...]

Tags: Ef's, Husband, Just, Should, What

A little about my past. I was on the internet a few month ago and spotted a woman on the “Hot or Not” site, I knew right then that she was a special filly. I finally had several emails with her and we decided that it would be convenient if I moved in the illegal [...]

Tags: Ef's, Husband, Just, Should, What

A little about my past. I was on the internet a few month ago and spotted a woman on the “Hot or Not” site, I knew right then that she was a special filly. I finally had several emails with her and we decided that it would be convenient if I moved in the illegal [...]

Tags: Ef's, Husband, Just, Should, What

A little about my past. I was on the internet a few month ago and spotted a woman on the “Hot or Not” site, I knew right then that she was a special filly. I finally had several emails with her and we decided that it would be convenient if I moved in the illegal [...]

Tags: Ef's, Husband, Just, Should, What

Please do not make jokes on this, this is really a problem for me. Sometimes (not always) when making love I might just be too tight to be penetrated, or penetration can be painful…and all this can happen when I am helthy, super relaxed, super arous and with a person who with I feel great [...]

Tags: Just, Tight, Tihght, When

A little about my past. I was on the internet a few month ago and spotted a woman on the “Hot or Not” site, I knew right then that she was a special filly. I finally had several emails with her and we decided that it would be convenient if I moved in the illegal [...]

Tags: Ef's, Husband, Just, Should, What

What do the people think about this news just out?

Tags: 3000, Adds, Centrifuges, Facility, Iran, Just, More, News, There, Uranium