Laboratory Instruments

Refering to Cheng, H.R., and Jiang, N., (2006) “Extremely rapid extraction of DNA from bacteria and yeast”, Biotechnology Letters, 28: 55-59. I followed most of the protocol, except that I used P:C:I (25:24:1) instead of tris-saturated phenol. 1. 1ml of culture, centrifuged, washed with STE buffer twice. 2. Resuspend cell pellets with TE buffer 3. [...]

Tags: B.subtilis, Can't, Culture, Extract, Just

Refering to Cheng, H.R., and Jiang, N., (2006) “Extremely rapid extraction of DNA from bacteria and yeast”, Biotechnology Letters, 28: 55-59. I followed most of the protocol, except that I used P:C:I (25:24:1) instead of tris-saturated phenol. 1. 1ml of culture, centrifuged, washed with STE buffer twice. 2. Resuspend cell pellets with TE buffer 3. [...]

Tags: B.subtilis, Can't, Culture, Extract, Just